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B cell line secreting monoclonal Abs with known sequences

#1

Hi,

Could you suggest a B cell line which secretes monoclonal antibodies with known sequence? Thank you.

#2

I don’t know your exact application, but is there any reason not to use one of the standard hybridomas?

#3

We are after human cell line producing soluble antibodies with as much similarity as possible. Thank you and looking for further suggestions. As an example, Raji would work, but it doesn’t secrete antibodies, just BCR expression.

#4

We are after human cell line producing soluble antibodies with as much similarity as possible.

Apologies for the delayed response. Is this related to this posting? If not, what exactly do you mean with “as much similarity as possible”? Similarity to what?