Human monoclonal secreting Ab-producing cell line with known Ig DNA Sequence


#1

Hello,

My group is interested in performing experiments on human monoclonal cell-lines that secrete Ig and that have been sequenced (Sanger, DNA-Seq or even RNA-Seq). Could you suggest such cell lines?

Thank you.


B cell line secreting monoclonal Abs with known sequences
#2

If you need the antibody itself as well, you could try building a cell line with Sai Reddy’s PnP system.


#3

We would be interested in acquiring cell lines that are well studied. And we’re not interested in a specific antibody, just in the cell line’s property of secreting them (so that we can assay ab synthesis).


#4

I’m not that much into human myelomes, but is there anything wrong with these ones?