BIOMED-2 BCR Sequencing


I am looking to perform some B cell repertoire sequencing using a multiplex PCR approach. What are the most accepted primers sets available for HC and LC in the field at the moment? BIOMED2s?


I think this is the most widely used protocol.


Depends on what your goal is for the sequencing. the Biomed-2 primers flank multiple framework region and is optimized to sequence the CDR3 …