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BIOMED-2 BCR Sequencing

#1

I am looking to perform some B cell repertoire sequencing using a multiplex PCR approach. What are the most accepted primers sets available for HC and LC in the field at the moment? BIOMED2s?

#2

I think this is the most widely used protocol.

#3

Depends on what your goal is for the sequencing. the Biomed-2 primers flank multiple framework region and is optimized to sequence the CDR3 …