Calling paired alpha-beta chains from bulk RNA seq


#1

Hi B-T.CR folks,
Is there anyway (package or tool) to infer paired alpha and beta chains from bulk RNA-seq?
Using MIXCR, I have TRA, and TRB chains but not paired alpha-beta! is this doable?
Many thanks


#2

It’s bulk RNA seq data, so the pairing information has been lost. There are various sequencing methods that preserve the pairing, such as “single cell sequencing” and “paired chain sequencing”, but you have to use these experimental methods first on the biological samples. Otherwise, it’s not doable


#3

thanks, makes perfect sense.