Demultiplex leak in sequencing


#1

I think I observe this in one of the dataset I am processing. the rate is low, but can still very bad.

I wonder how it is handled. The machine is ion-torrent. seems everyone is using ilumina platform.

Best practice?

http://b-t.cr/t/5race-tcr-sequencing-protocol-douek-lab?source_topic_id=267

The protocol above mention dual-bar coding. But what if no dual-barcode is done, possible to fix?