Human TCR primers and PCR protocol


#1

Does anyone have experience with the BIOMED2 TCRB primers? Are these considered the best ones to use? Right now I just want to implement a feasibility study on new sample types to determine whether there is enough rearranged TCRB DNA present in the sample to make it worth it to spend the money for repertoire sequencing. So we will probably just do PCR and then run the product out on a gel to determine if we get right sized bands.

Any suggestions welcome. Thanks!